Test Species and Methods

(click on the images for videos of the testing organisms)

Freshwater Species

Water fleas (Ceriodaphnia dubia)



Water fleas (Daphnia magna)



Fathead minnows (Pimephales promelas)



Saltwater Species


Mysid shrimp (Americamysis bahia)



Inland silversides (Menidia beryllina)


ETS is certified by NC DEQ, SC DHEC and KY EEC for the following tests and methods.

Daphnid, Ceriodaphnia dubia, Acute EPA 2002.0
Daphnid, Ceriodaphnia dubia, Survival and Reproduction EPA 1002.0
Daphnid, Ceriodaphnia dubia, Survival and Reproduction
by North Carolina Modifications (Pass/Fail or Phase II Tests)
EPA 1002.0
Fathead Minnow, Pimephales promelas, Acute EPA 2000.0
Fathead Minnow, Pimephales promelas, Larval Survival and Growth EPA 1000.0
Mysid Shrimp, Americamysis bahia, Acute EPA 2007.0
Mysid Shrimp, Americamysis bahia, Survival, Growth, and Fecundity EPA 1007.0
Inland Silverside, Menidia beryllina, Acute EPA 2006.0
Inland Silverside, Menidia beryllina, Larval Survival and Growth EPA 1006.0
EPA Acute Manual:  EPA-821-R-02-012, Methods 2000.0, 2002.0, 2006.0, 2007.0.

EPA Freshwater Chronic Manual:  EPA-821-R-02-013, Methods 1000.0, 1002.0

EPA Saltwater Chronic Manual:  EPA-821-R-02-014, Methods 1006.0, 1007.0

North Carolina Pass/Fail Chronic Modification

North Carolina Phase II Chronic Modification

Additional tests and species may be available upon request.

ETS performs research projects to identify the source of toxicants (Toxicity Identification Evaluations or TIE) and to evaluate changes in wastewater and industrial treatment operations.


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